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pCMV/myc/cyto载体质粒
产品名称: pCMV/myc/cyto载体质粒
英文名称: pCMV/myc/cyto载体质粒
产品编号: BiovectorPCMVMC
产品价格: 0
产品产地: Biovector Inc. USA
品牌商标: Biovector, Addgene, ATCC, Invi
更新时间: null
使用范围: null
Biovector Co.,LTD
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Order ID |
Name |
Description |
BiovectorPCMVMC |
pCMV/myc/cyto |
pCMV/myc/cyto 15uL DNA. AmpR.Strage:-20℃ |
Standard heat-shock transformation of chem ically compe tent bacteria
- Take competent cells out of -80°C and thaw on ice.
- Take agar plates (containing the appropriate antibiotic) out of 4°C to warm up to room temperature or place in 37°C incubator.
- Mix 5μl of DNA into 100μL competent cells in a microcentrifuge or falcon tube. GENTLY mix by flicking the bottom of the tube with your finger a few times.
- Place the competent cell/DNA mixture on ice for 20-30min.
- Heat shock each transformation tube by placing the bottom 2/3 of the tube into a 42°C water bath for 42 seconds. Put the tubes back on ice for 2 min.
- Add 250-500μl SOC media (without antibiotic) and grow in 37°C shaking incubator for 45min.
- Plate all of the transformation onto a 10c, m LB agar plate containing the appropriate antibiotic.
- Incubate plates at 37°C overnight.
载体信息:
载体名称: |
pCMV/myc/cyto |
质粒类型: |
哺乳细胞表达载体;亚细胞定位 |
高拷贝/低拷贝: |
高拷贝 |
启动子: |
CMV |
克隆方法: |
多克隆位点,限制性内切酶 |
载体大小: |
4883 bp |
5 测序引物及序列: |
CMV Forward CGCAAATGGGCGGTAGGCGTG |
3 测序引物及序列: |
-- |
载体标签: |
c-myc |
载体抗性: |
氨苄青霉素(Ampicillin) |
筛选标记: |
Neomycin |
备注: |
TUNEology 波长可调检测卡盒-->
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